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Project 3 Prof. Andreas Bechthold

Studies on F420–dependent oxygenases involved in the biosynthesis of polyketides

Combinatorial biosynthesis can refer to any strategy for the genetic engineering of natural product biosynthesis to obtain new molecules, including the use of genetics for medicinal chemistry. Tailoring enzymes as part of natural compound biosynthesis are of great importance since the individual decoration of a compound often has serious impact on the activity of a substance. In this project we are focusing our work on oxygenases and glycosyltransferases and the cofactors used by these enzymes. We are aiming at understanding the reaction mechanisms of tailoring enzymes and at using the corresponding genes to generate novel molecules. In addition, the crystal structures of enzymes will be elucidated to learn about substrate binding and the mechanistic role of the cofactors aiming at altering substrate specificities. As an example, one project will be about the luciferase like monooxygenases RslO1 most probably catalyzing an unusual Baeyer-Villiger oxidation during rishirilid biosynthesis.





Prof. Dr. Andreas Bechthold

Institute of Pharmaceutical Sciences

Pharmaceutical Biology and Biotechnology

Stefan-Meier-Str. 19, 79104 Freiburg

Phone: +49 (0) 761 203-8371

Fax: +49 (0) 761 203-8383

email: andreas.bechthold@pharmazie.uni-freiburg.de



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